6 Factors Affecting Enzyme Activity Lab
Hydrogen peroxide (H
2
O
2
) is a product of alcohol detoxification.
H
2
O
2
is produced in
peroxisomes
found in all eukaryotic cells, including liver cells, where the detoxification takes place.
Converted to water by
Hydrogen peroxidase
: 2H
2
O
2
-> 2H
2
O + O
2
; the O
2
can be collected and measured in this lab.
Enzymes act by lowering the
activation
energy of chemical reactions, and have
optimal
temperature and pH conditions.
A: Effect of temperature
Cut strips of filter paper 5 cm long to absorb enzyme.
Tray
setup.
Record the 3 actual temperatures
in the columns.
When entering data, the volume should increase as time intervals increase, until it levels off.
Graph
the results. Try to put all 3 treatments for each section on one graph, so each graph should be 3 line graphs.
Which is the independent variable (X axis)?
B: Effect of enzyme concentration: 1/2 strip, 1 strip, 2 strips.
C: Effect of pH: use 50% HCL and 50% NaOH, measure pH with pH paper.
Index
Lab notes made Oct 13, 2008 by
Peter Chen
