A haploid set of human chromosomes with 3.2 billion base pairs was sequenced in 2003.
- Cut the DNA into overlapping fragments with _FIG_ch20/restriction.html endonucleases.
- Clone the fragments in plasmid or phage _FIG_ch20/cloning.html vectors.
- Sequence each fragment with _FIG_ch20/dideoxy.html dideoxyribonucleotides.
- Order the sequences into one overall sequence with computer software.