APPLICATION
The sequencing of nucleotide fragments up to 800 base pairs can be determined by specialized machines that separate the
labled reaction products by length.
TECHNIQUE Each strand stars with the same primer and ends with a di-deoxyribonucleotide ( ddNTP ). Incorporation of ddNTP terminates a growing DNA strand because it lacks a 3'-OH to attach the next nucleotide. By tagging the ddNTP with different fluorescent dyes, the oroginal nucleotide sequence can be determined.
RESULTS The color of the fluorescent tag on each strand identifies the nucleotide at its end.
