DNA microarray.
- Isolate mRNA
from a tissue sample.
- Make cDNA
by reverse-transcription, using fluorescent nucleotides.
- Each spot of the microarray contains DNA
of known sequence from known alleles.
- Let the cDNA
hybridize (base-pair with complementary sequences) with DNA
on the microarray
The fluorescence patterns of hybridized DNA
reveals gene expression of the tissue.
Testing for alleles: 